Diagnostic expression of the cotton pink bollworm Pectinophora gossypiella (Saunders) Ace1 exposed to some insecticide by the real-time PCR

The Pink Bollworm Pectinophora gossypiella (Saunders) is a devastating pest in Egyptian cotton field. Insecticide control failure may be happen due to its biological features of hide inside bolls and rarely exposed insecticide application. Thus, bulk infested with larvae collected from fields Sharkia Benysuef governorate. Moreover, py, Op sub lethal concentrations laboratory bioassay. Toxicity as LC50, LC90 the slope, RR was completed. Whereas common target site OP pyrethroid acetylcholinesterase enzyme. gene Ace1 encoding AChE relative expression investigated using molecular marker real-time PCR procedures via steps generating data RNA isolation characterization, cDNA synthesis, then normalization factors, Ct attained analyzed 2-ΔΔCT method quantify quantitatively treated tissue extracted. Data showed most expressed ratio samples cypermethrin followed by deltamethrin compared least malathion chlorpyrifos, thus, effective were according ct values. Hierarchical clustering combined Heat map principle component analysis based on various systems algorithm distance measures Validation reference addition stability three optimal finders that genes having lower mean weights considered transcriptionally stable ideal genes.